ACTION OF NEUROHYPOPHYSEAL GRANULE LYS-ARG ENDOPEPTIDASE ON SYNTHETIC POLYPEPTIDES COMPRISING THE PROCESSING SEQUENCE OF PROVASOPRESSIN-NEUROPHYSIN

被引：3

作者：

MICHEL, G ^{[1
]}

ROUILLE, Y ^{[1
]}

CHAUVET, J ^{[1
]}

ACHER, R ^{[1
]}

机构：

[1] UNIV PARIS 06,BIOL CHEM LAB,F-75006 PARIS,FRANCE

来源：

BIOSCIENCE REPORTS | 1994年 / 14卷 / 04期

关键词：

GRANULE LYS-ARG ENDOPEPTIDASE; NEUROHYPOPHYSEAL GRANULES; VASOPRESSINYL-PEPTIDE PROCESSING;

D O I：

10.1007/BF01200246

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neurohypophysial granule Ca2+-dependent endopeptidases have been allowed to act on synthetic polypeptides derived from the N-terminal sequence of bovine provasopressin-neurophysin, namely vasopressinyl-glycyl-lysyl-arginyl-alanylamide and vasopressinyl-glycyl-lysyl-arginyl-alany-methionyl-serinamide. Membrane-bound enzymes have been used at pH5.5 for 16 hr at 37 degrees C. Products have been identified by high-pressure liquid chromatography (HPLC) and by mass spectrometry performed on substances isolated by HPLC. With both substrates, vasopressinyl-Gly-lys-Arg(OH) has been identified as a product confirming the Lys-Arg specificity previously observed an small peptide fluorogenic substrates. Cleavage yields, however, appear low suggesting that some factors are missing, for example a targeting action of the precursor neurophysin domain to the granule membrane.

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收藏

页码：171 / 178

页数：8

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