EFFECT OF ALBUMIN ON OLEIC-ACID LYMPHATIC ABSORPTION IN RATS

1. 1. The aim of this study was to investigate how fatty acid absorption was affected when exogenous fatty acids were complexed with albumin in absence of bile. Experiments were carried out in vivo, in order to study overall absorption processes. 2. 2. An equimolar mixture of 14C oleic acid, palmitic acid and monopalmitin was infused intraduodenally in bile- and pancreatic juice-diverted rats. 3. 3. Lipids were emulsified with either sodium taurocholate or fatty acids complexed with albumin. 4. 4. Lymphatic lipid output was compared during the 6 hr following infusion of 90 μmol of the radioactive lipid mixture. 5. 5. Lymphatic radioactive lipid recovery was significantly decreased by albumin. 6. 6. Only 17% of the infused radioactivity was recovered in lymph when fatty acids were complexed with albumin against 37% when lipids were emulsified with sodium taurocholate. 7. 7. Unrecovered lymph radioactivity was found at the distal part of intestine. Moreover, albumin significantly decreased lymph flow. 8. 8. We conclude that undigested albumin acted at the luminal level of lipid absorption processes and specifically decreased fatty acid uptake. © 1990.