CHARACTERIZATION OF PEPTIDES IN SEA-ANEMONE VENOM COLLECTED BY A NOVEL PROCEDURE

被引：52

作者：

MALPEZZI, ELA

DEFREITAS, JC

MURAMOTO, K

KAMIYA, H

机构：

[1] UNIV SAO PAULO,DEPT GEN PHYSIOL,INST BIOSCI,RUA MATAO,TRAVESSA 14 101,BR-05508 SAO PAULO,BRAZIL

[2] KITASATO UNIV,SCH FISHERIES SCI,SANRIKU,IWATE 02201,JAPAN

[3] UNIV SAO PAULO,INST MARINE BIOL,BR-05508 SAO PAULO,BRAZIL

来源：

TOXICON | 1993年 / 31卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

D O I：

10.1016/0041-0101(93)90220-D

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Peptide neurotoxins were isolated from the venom obtained by electrical stimulation of the sea anemone Bunodosoma caissarum. This technique allows almost pure venom to be collected, and the animals to survive. Three neurotoxins (assayed on crustacean nerves) were isolated by gel filtration and reversed-phase high performance liquid chromatography. Hemolysins were also detected in the venom. The amino acid sequence of a major neurotoxin BcIII was determined. BcIII has 48 amino acid residues with six half-cystine residues. This sequence has homology with the type 1 long sea anemone neurotoxins. Two minor toxins (BcI and II) have similar amino acid composition and amino-terminal sequences to BcIII.

引用

页码：853 / 864

页数：12

共 27 条

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