IDENTIFICATION OF 2 CELL-CYCLE-REGULATED GENES AFFECTING THE BETA-1,3-GLUCAN CONTENT OF CELL-WALLS IN SACCHAROMYCES-CEREVISIAE

被引：123

作者：

RAM, AFJ ^{[1
]}

BREKELMANS, SSC ^{[1
]}

OEHLEN, LJWM ^{[1
]}

KLIS, FM ^{[1
]}

机构：

[1] ROCKEFELLER UNIV,NEW YORK,NY 10021

来源：

FEBS LETTERS | 1995年 / 358卷 / 02期

关键词：

GAS1; GGP1; CALCOFLUOR WHITE; CELL WALL; CELL CYCLE ARREST; P-GLUCAN;

D O I：

10.1016/0014-5793(94)01418-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Calcofluor white-hypersensitive mutants cwh52 and cwh53 are severely reduced in beta 1,3-glucan. CWH52 was equivalent to GAS1. CWH53 represented a new gene, located on the right arm of chromosone XII, and predicted to encode a 215 kDa protein with multiple transmembrane domains. The transcription of CWH53 was cell cycle-dependent and, similar to GAS1/CWH52, increased in late G(1), indicating that the formation of beta-glucan is cell cycle-regulated. Further, in some mutant alleles of both gas1/cwh52 and cwh53 lethal concentrations of Calcofluor induced growth arrest at a specific phase of the cell cycle.

引用

页码：165 / 170

页数：6

共 53 条

[11] A SACCHAROMYCES-CEREVISIAE MUTANT WITH ECHINOCANDIN-RESISTANT 1,3-BETA-D-GLUCAN SYNTHASE [J].