RAPID, SENSITIVE METHOD FOR ACCURATE DETERMINATION OF THE LECITHIN-SPHINGOMYELIN RATIO BY THIN-LAYER CHROMATOGRAPHY AND REFLECTANCE SPECTROFLUOROMETRY

被引：10

作者：

NG, DS ^{[1
]}

BLASS, KG ^{[1
]}

机构：

[1] UNIV REGINA,DEPT CHEM,REGINA S4S 0A2,SASKATCHEWAN,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 163卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)81166-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly reproducible thin-layer chromatographic procedure has been developed for accurate determination of the lecithin/sphingomyelin ratio. Two interfering compounds, phosphatidyl inositol and phosphatidyl serine, have been investigated and eliminated by adsorption onto DEAE-cellulose. A uniform fluorescence staining procedure employing 2′,7′-dichlorofluorescein has been developed. Accurate quantitation was performed by direct measurement of the reflected fluorescence intensity of the lecithin and sphingomyelin fluorophore spots with a spectrofluorometer equipped with a thin-layer scanning attachment. Stability and reproducibility studies are reported. © 1979.

引用

页码：37 / 46

页数：10

共 13 条

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