EXPRESSION OF THE SARCOPLASMIC-RETICULUM CA2+-ATPASE IN YEAST

被引：37

作者：

CENTENO, F

DESCHAMPS, S

LOMPRE, AM

ANGER, M

MOUTIN, MJ

DUPONT, Y

PALMGREN, MG

VILLALBA, JM

MOLLER, JV

FALSON, P

LEMAIRE, M

机构：

[1] CENS, CEA, DBCM, BIOPHYS PROT & MEMBRANES SECT, F-91191 GIF SUR YVETTE, FRANCE

[2] CEA, CNRS, F-91191 GIF SUR YVETTE, FRANCE

[3] UNIV PARIS 11, CTR ORSAY, CNRS, URA 1131, F-91405 ORSAY, FRANCE

[4] CEG, CEA,DBMS,BIOPHYS MOLEC & CELLULAIRE LAB,CNRS, URA 520, F-38041 GRENOBLE, FRANCE

[5] ROYAL VET & AGR UNIV, DEPT PLANT BIOL, DK-1871 FREDERIKSBERG C, DENMARK

[6] FAC CIENCIAS SPAIN, DEPT BIOL CELULAR, E-14004 CORDOBA, SPAIN

[7] AARHUS UNIV, INST BIOPHYS, DANISH BIOMEMBRANE RES CTR, DK-8000 AARHUS C, DENMARK

来源：

FEBS LETTERS | 1994年 / 354卷 / 01期

关键词：

D O I：

10.1016/0014-5793(94)01104-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here an easy system for the production of mg amounts of the rabbit Ca2+-ATPase SERCA la in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca2+-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.

引用

页码：117 / 122

页数：6

共 39 条

[1] 2 CA-2+ ATPASE GENES - HOMOLOGIES AND MECHANISTIC IMPLICATIONS OF DEDUCED AMINO-ACID-SEQUENCES [J].