DUCK HEPATITIS-B VIRUS - A MODEL TO ASSESS EFFICACY OF DISINFECTANTS AGAINST HEPADNAVIRUS INFECTIVITY

被引：31

作者：

MURRAY, SM ^{[1
]}

FREIMAN, JS ^{[1
]}

VICKERY, K ^{[1
]}

LIM, D ^{[1
]}

COSSART, YE ^{[1
]}

WHITELEY, RK ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT INFECT DIS,SYDNEY,NSW 2006,AUSTRALIA

来源：

EPIDEMIOLOGY AND INFECTION | 1991年 / 106卷 / 03期

关键词：

D O I：

10.1017/S0950268800067480

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The efficacy of three proprietary glutaraldehyde disinfectants and their component bases was assessed using the duck hepatitis B virus (DHBV) model. Inactivation of infectivity of undiluted serum containing 10(6.8) ID50/ml DHBV was assessed after a mixture with an equal volume of disinfectant had stood at room temperature for 10 min. A dried spill of infectious serum was simulated using sterile filter paper disks, saturated with serum containing DHBV, dried and then exposed to test disinfectant for 10 min. Residual infectivity, and hence the reduction in virus titre, was determined by inoculation of dilutions of the treated samples into 1-day-old ducklings. A greater than 3 log10 reduction in virus titre could be demonstrated for the disinfectants as well as for some of their component bases. Disinfectant activity varied according to the method of viral presentation but a reduction of exposure time from 10 to 2.5 min did not diminish activity. The experimental protocol permits a comparative and quantitative assessment of the efficacy of both established and new disinfectants.

引用

页码：435 / 443

页数：9

共 23 条

[11]

MARION PL, 1983, CURR TOP MICROBIOL, V105, P99

[12]

MARION PL, 1988, PROG MED VIROL, V35, P43

[13]

MARION PL, 1984, P NAT ACAD SCI, V81, P889

[14] ASYMMETRIC REPLICATION OF DUCK HEPATITIS-B VIRUS-DNA IN LIVER-CELLS - FREE MINUS-STRAND DNA [J].

MASON, WS ;

ALDRICH, C ;

SUMMERS, J ;

TAYLOR, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (13) :3997-4001

[15]

OMATA M, 1983, GASTROENTEROLOGY, V85, P260

[16]

PATTISON CP, 1974, JAMA-J AM MED ASSOC, V230, P854

[17]

REED L. J., 1938, AMER JOUR HYG, V27, P493

[18]

Russell A D, 1976, Prog Med Chem, V13, P271, DOI 10.1016/S0079-6468(08)70140-1

[19]

SCOTT EM, 1983, DISINFECTION STERILI, P981

[20]

SPRENGEL R, 1987, VIRUS DISEASE LABORA, V9

← 1 2 3 →