DNA-FINGERPRINTING IN RICE USING OLIGONUCLEOTIDE PROBES SPECIFIC FOR SIMPLE REPETITIVE DNA-SEQUENCES

被引：55

作者：

RAMAKISHANA, W ^{[1
]}

LAGU, MD ^{[1
]}

GUPTA, VS ^{[1
]}

RANJEKAR, PK ^{[1
]}

机构：

[1] NATL CHEM LAB, DEPT BIOCHEM SCI, PLANT MOLEC BIOL UNIT, POONA 411008, MAHARASHTRA, INDIA

来源：

THEORETICAL AND APPLIED GENETICS | 1994年 / 88卷 / 3-4期

关键词：

DNA FINGERPRINTING; GENOTYPE IDENTIFICATION; OLIGO PROBES; RICE;

D O I：

10.1007/BF00223651

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In this report we describe the use of five oligonucleotide probes, namely (GATA)(4), (GACA)(4), (GGAT)(4), (GAA)(6) and (CAC)(5), to reveal highly polymorphic DNA regions in rice. With each of the oligonucleotide probes, the level of polymorphism was high enough to distinguish several rice genotypes. Moreover, individual plants of one cultivar showed the same cultivar-specific DNA fingerprint. The multilocus fingerprint patterns were somatically stable. Our study demonstrates that microsatellite-derived DNA fingerprints are ideally suited for the identification of rice genotypes. As the majority of the probes detected a high level of polymorphism, they can be very useful in monitoring and aiding gene introgression from wild rice into cultivars.

引用

页码：402 / 406

页数：5

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