THE ISOCITRATE LYASE GENE OF CUCUMBER - ISOLATION, CHARACTERIZATION AND EXPRESSION IN COTYLEDONS FOLLOWING SEED-GERMINATION

被引：21

作者：

REYNOLDS, SJ ^{[1
]}

SMITH, SM ^{[1
]}

机构：

[1] UNIV EDINBURGH,INST CELL & MOLEC BIOL,EDINBURGH EH9 3JH,MIDLOTHIAN,SCOTLAND

来源：

PLANT MOLECULAR BIOLOGY | 1995年 / 27卷 / 03期

关键词：

CUCUMIS SATIVUS; GENE EXPRESSION; GLYOXYLATE CYCLE; GLYOXYSOME; ISOCITRATE LYASE; SEED GERMINATION;

D O I：

10.1007/BF00019316

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cucumber (Cucumis sativus L.) genome contains only a single gene encoding the glyoxylate cycle enzyme isocitrate lyase (ICL). The cucumber icl gene has been isolated and sequenced, revealing only two small introns. The predicted amino acid sequence is more than 85%; identical to ICL from other higher plants, and contains the C-terminal tripeptide Ser-Arg-Met which resembles a peroxisomal targeting sequence. The icl gene is coordinately expressed with the malate synthase (ms) gene after seed germination in both the light and the dark, suggesting that these genes may contain similar DNA elements regulating transcription. The start of transcription of the icl gene was determined and the DNA sequences upstream compared with the region of the ms gene promoter known to regulate transcription. This comparison revealed a highly conserved DNA sequence at similar positions in each gene.

引用

页码：487 / 497

页数：11

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