DETECTION OF MURAMIC ACID IN A CARBOHYDRATE FRACTION OF HUMAN SPLEEN

被引:36
作者
HOIJER, MA [1 ]
MELIEF, MJ [1 ]
VANHELDENMEEUWSEN, CG [1 ]
EULDERINK, F [1 ]
HAZENBERG, MP [1 ]
机构
[1] STICHTING SAMENWERKING DELFTSE ZIEKENHUIZEN,CENTRUM DIAGNOST,DELFT,NETHERLANDS
关键词
D O I
10.1128/IAI.63.5.1652-1657.1995
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In previous studies, we showed that peptidoglycan polysaccharides from anaerobic bacteria normally present in the human gut induced severe chronic joint inflammation in rats. Our hypothesis is that peptidoglycan from the gut flora is involved in perpetuation of idiopathic inflammation. However, in the literature, the presence of peptidoglycan or subunits like muramyl peptides in blood or tissues is still a matter of debate. We were able to stain red pulp macrophages in all six available human spleens by immunohistochemical techniques using a monoclonal antibody against gut flora-derived antigens. Therefore, these human spleens were extracted, and after removal of most of the protein, the carbohydrate fraction was investigated for the presence of muramic acid, an amino sugar characteristic for peptidoglycan. Using three different methods for detection of muramic acid, we found a mean of 3.3 mu mol of muramic acid with high-pressure liquid chromatography, 1,9 mu mol with a colorimetric method for detection of lactate, and 0.8 mu mol with an enzymatic method for detection of D-lactate per spleen (D-lactate is a specific group of the muramic acid molecule). It is concluded that peptidoglycan is present in human spleen not as small muramyl peptides as were previously searched for by other investigators but as larger macromolecules probably stored in spleen macrophages.
引用
收藏
页码:1652 / 1657
页数:6
相关论文
共 30 条
[21]  
SEVERIJNEN AJ, 1989, J RHEUMATOL, V16, P1061
[22]   CHRONIC ARTHRITIS INDUCED IN RATS BY CELL-WALL FRAGMENTS OF EUBACTERIUM SPECIES FROM THE HUMAN INTESTINAL FLORA [J].
SEVERIJNEN, AJ ;
VANKLEEF, R ;
HAZENBERG, MP ;
VANDEMERWE, JP .
INFECTION AND IMMUNITY, 1990, 58 (02) :523-528
[23]  
SEVERIJNEN AJ, 1990, BRIT J RHEUMATOL, V29, P433
[24]   D-LACTIC ACID MEASUREMENTS IN THE DIAGNOSIS OF BACTERIAL-INFECTIONS [J].
SMITH, SM ;
ENG, RHK ;
CAMPOS, JM ;
CHMEL, H .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (03) :385-388
[25]   INDUCTION OF RELEASE OF TUMOR-NECROSIS-FACTOR FROM HUMAN MONOCYTES BY STAPHYLOCOCCI AND STAPHYLOCOCCAL PEPTIDOGLYCANS [J].
TIMMERMAN, CP ;
MATTSSON, E ;
MARTINEZMARTINEZ, L ;
DEGRAAF, L ;
VANSTRIJP, JAG ;
VERBRUGH, HA ;
VERHOEF, J ;
FLEER, A .
INFECTION AND IMMUNITY, 1993, 61 (10) :4167-4172
[26]   ALKALI-CATALYZED ELIMINATION OF D-LACTIC ACID FROM MURAMIC ACID AND ITS DERIVATIVES AND DETERMINATION OF MURAMIC ACID [J].
TIPPER, DJ .
BIOCHEMISTRY, 1968, 7 (04) :1441-&
[27]  
TOMASIC J, 1986, BIOL PROPERTIES PEPT, P203
[28]  
TONDOME Y, 1992, INT ARCH ALLERGY IMM, V97, P301
[29]  
WEIDEL W, 1964, ADV ENZYMOL REL S BI, V26, P193
[30]   FACTORS AFFECTING COMPLEMENT ACTIVATION BY STAPHYLOCOCCUS-AUREUS CELL-WALLS, THEIR COMPONENTS, AND MUTANTS ALTERED IN TEICHOIC-ACID [J].
WILKINSON, BJ ;
KIM, Y ;
PETERSON, PK .
INFECTION AND IMMUNITY, 1981, 32 (01) :216-224