MATHEMATICAL-MODELING OF SINGLE-LINE FLOW-INJECTION ANALYSIS SYSTEMS WITH SINGLE-LAYER ENZYME ELECTRODE DETECTION .3. EXPERIMENTAL-VERIFICATION OF THE MODEL

Glucose and urea electrodes, prepared by two different enzyme immobilization techniques and used as detectors in a single-line flow-injection manifold, were experimentally investigated for elucidating the influence of their most important parameters, i.e., the initial substrate concentration in the sample, the enzyme concentration in the reaction layer and its thickness and the buffer concentration, on the output signal. The results obtained were compared with the theoretical predictions based on simulations of the model for single-line flow-injection systems with single-layer enzyme electrode detection. The good qualitative agreement which was observed is a convincing experimental verification of this model and of the guidelines for the production of flow-through biocatalytic electrodes with optimum design based upon it.