AUTOIMMUNE ADDISONS-DISEASE - ANALYSIS OF AUTOANTIBODY BINDING-SITES ON HUMAN STEROID 21-HYDROXYLASE

被引：38

作者：

WEDLOCK, N

ASAWA, T

BAUMANNANTCZAK, A

SMITH, BR

FURMANIAK, J

机构：

[1] Endocrine Immunology Unit, 7th floor Medicine, University of Wales College of Medicine, Cardiff, CF4 4XN, Heath Park

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

21-HYDROXYLASE; ADDISONS DISEASE; ADRENAL AUTOANTIBODY; AUTOANTIBODY EPITOPE;

D O I：

10.1016/0014-5793(93)80497-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human steroid 21 -hydroxylase (21-OH) expressed in an in vitro translation system was found to react specifically with adrenal autoantibodies from patients with Addison's disease. The epitopes on 21-OH which reacted with autoantibodies were studied by incorporating a series of terminal and internal deletions into the 21-OH gene and analysing the expressed proteins by Western blotting. N-Terminal deletions up to amino acid 280 had no effect on autoantibody binding whereas a series of C-terminal deletions and truncations (amino acids 281-494) showed marked effects. Our results indicate that a central segment (281-379) and a C-terminal segment (380-494) of 21-OH interact to form at least one major autoantibody binding site.

引用

页码：123 / 126

页数：4