TARGETED EXPRESSION OF TETANUS TOXIN LIGHT-CHAIN IN DROSOPHILA SPECIFICALLY ELIMINATES SYNAPTIC TRANSMISSION AND CAUSES BEHAVIORAL DEFECTS

被引：674

作者：

SWEENEY, ST

BROADIE, K

KEANE, J

NIEMANN, H

OKANE, CJ

机构：

[1] UNIV CAMBRIDGE,DEPT ZOOL,CAMBRIDGE CB2 3EH,ENGLAND

[2] BUNDESFORSCH ANSTALT VIRUSKRANKHEITEN TIERE,D-72001 TUBINGEN,GERMANY

来源：

NEURON | 1995年 / 14卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0896-6273(95)90290-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Tetanus toxin cleaves the synaptic vesicle protein synaptobrevin, and the ensuing loss of neurotransmitter exocytosis has implicated synaptobrevin in this process. To further the study of synaptic function in a genetically tractable organism and to generate a tool to disable neuronal communication for behavioural studies, we have expressed a gene encoding tetanus toxin light chain in Drosophila. Toxin expression in embryonic neurons removes detectable synaptobrevin and eliminates evoked, but not spontaneous, synaptic vesicle release. No other developmental or morphological defects are detected. Correspondingly, only synaptobrevin (n-syb), but not the ubiquitously expressed syb protein, is cleaved by tetanus toxin in vitro. Targeted expression of toxin can produce specific behavioral defects; in one case, the olfactory escape response is reduced.

引用

页码：341 / 351

页数：11

共 45 条

[31] SYNAPTIC FUNCTION IS IMPAIRED BUT NOT ELIMINATED IN C-ELEGANS MUTANTS LACKING SYNAPTOTAGMIN
NONET, ML
GRUNDAHL, K
MEYER, BJ
RAND, JB
[J]. CELL, 1993, 73 (07) : 1291 - 1305
[32] INHIBITION OF AXONAL GROWTH BY SNAP-25 ANTISENSE OLIGONUCLEOTIDES IN-VITRO AND IN-VIVO
OSENSAND, A
CATSICAS, M
STAPLE, JK
JONES, KA
AYALA, G
KNOWLES, J
GRENNINGLOH, G
CATSICAS, S
[J]. NATURE, 1993, 364 (6436) : 445 - 448
[33] NEUROTRANSMITTER RELEASE IS BLOCKED INTRACELLULARLY BY BOTULINUM NEUROTOXIN, AND THIS REQUIRES UPTAKE OF BOTH TOXIN POLYPEPTIDES BY A PROCESS MEDIATED BY THE LARGER CHAIN
POULAIN, B
TAUC, L
MAISEY, EA
WADSWORTH, JDF
MOHAN, PM
DOLLY, JO
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) : 4090 - 4094
[34] SHORT-TERM SYNAPTIC PLASTICITY IS ALTERED IN MICE LACKING SYNAPSIN-I
ROSAHL, TW
GEPPERT, M
SPILLANE, D
HERZ, J
HAMMER, RE
MALENKA, RC
SUDHOF, TC
[J]. CELL, 1993, 75 (04) : 661 - 670
[35] TETANUS AND BOTULINUM-B NEUROTOXINS BLOCK NEUROTRANSMITTER RELEASE BY PROTEOLYTIC CLEAVAGE OF SYNAPTOBREVIN
SCHIAVO, G
BENFENATI, F
POULAIN, B
ROSSETTO, O
DELAURETO, PP
DASGUPTA, BR
MONTECUCCO, C
[J]. NATURE, 1992, 359 (6398) : 832 - 835
[36] PROTEOLYTIC CLEAVAGE OF SYNTHETIC FRAGMENTS OF VESICLE-ASSOCIATED MEMBRANE-PROTEIN, ISOFORM-2 BY BOTULINUM TYPE-B NEUROTOXIN
SHONE, CC
QUINN, CP
WAIT, R
HALLIS, B
FOOKS, SG
HAMBLETON, P
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 217 (03): : 965 - 971
[37] SNAP RECEPTORS IMPLICATED IN VESICLE TARGETING AND FUSION
SOLLNER, T
WHITEHART, SW
BRUNNER, M
ERDJUMENTBROMAGE, H
GEROMANOS, S
TEMPST, P
ROTHMAN, JE
[J]. NATURE, 1993, 362 (6418) : 318 - 324
[38] SPRADLING AC, 1986, DROSOPHILA PRACTICAL, P60
[39] A SYNAPTIC VESICLE MEMBRANE-PROTEIN IS CONSERVED FROM MAMMALS TO DROSOPHILA
SUDHOF, TC
BAUMERT, M
PERIN, MS
JAHN, R
[J]. NEURON, 1989, 2 (05) : 1475 - 1481
[40] VAMP-1 - A SYNAPTIC VESICLE-ASSOCIATED INTEGRAL MEMBRANE-PROTEIN
TRIMBLE, WS
COWAN, DM
SCHELLER, RH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) : 4538 - 4542

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