PRODUCTION OF HIV-1 VPU WITH PYEULCBX, A CONVENIENT VECTOR FOR THE PRODUCTION OF NONFUSED PROTEINS IN YEAST

被引：13

作者：

MACREADIE, IG

FAILLA, P

HORAITIS, O

AZAD, AA

机构：

[1] Biomolecular Research Institute, Parkville, 3052, Victoria

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 08期

关键词：

D O I：

10.1007/BF01021634

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Site directed mutagenesis was employed to modify a vector previously constructed for the convenient, copper-inducible production of fusion proteins in the yeast Saccharomyces cerevisiae. The resulting vector, pYEULCBX, remains versatile and now permits non-fused proteins to be produced. The vpu gene from HIV-1 was expressed as an example.

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页码：639 / 642

页数：4

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