APP EXPRESSION IN PRIMARY NEURONAL CELL-CULTURES FROM P6 MICE DURING IN-VITRO DIFFERENTIATION

被引:11
作者
DICHGANS, M
MONNING, U
KONIG, G
SANDBRINK, R
MASTERS, CL
BEYREUTHER, K
机构
[1] UNIV HEIDELBERG,CTR MOLEC BIOL,D-69120 HEIDELBERG,GERMANY
[2] UNIV MELBOURNE,DEPT PATHOL,PARKVILLE,VIC 3052,AUSTRALIA
来源
DEMENTIA | 1993年 / 4卷 / 06期
关键词
ALZHEIMERS DISEASE; AMYLOID PROTEIN PRECURSOR; CEREBELLAR NEURONS; SIALIC ACID;
D O I
10.1159/000107337
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Primary neuronal cell cultures from P6 mice were investigated in order to study amyloid protein precursor (APP) gene expression in differentiating neurons. Cerebellar granule cells which strongly express APP 695 allowed the identification of three distinct isoforms of neuronal APP 695. The high-molecular-weight form of APP 695 is sialylated. The expression pattern of neuronal APP 695 changes during in vitro differentiation. Sialylated forms become more abundant upon longer cultivation time. The secreted forms of sialylated, neuronal APP 695 are shown to comigrate with APP isolated from cerebrospinal fluid. We suggest that the different sialylation states of APP 695 may reflect the modulation of cell-cell and cell-substrate interactions during in vitro differentiation and regeneration.
引用
收藏
页码:301 / 307
页数:7
相关论文
共 43 条
[41]  
TRENKNER E, 1984, J NEUROSCI, V4, P2850
[42]  
VANNOSTRAND WE, 1989, NATURE, V341, P546
[43]   IDENTIFICATION, BIOGENESIS, AND LOCALIZATION OF PRECURSORS OF ALZHEIMERS-DISEASE A4 AMYLOID PROTEIN [J].
WEIDEMANN, A ;
KONIG, G ;
BUNKE, D ;
FISCHER, P ;
SALBAUM, JM ;
MASTERS, CL ;
BEYREUTHER, K .
CELL, 1989, 57 (01) :115-126