ENZYMATIC METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF ASPARTAME

被引:9
作者
DINCKAYA, E
CAGIN, M
TELEFONCU, A
机构
[1] Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova, Izmir
关键词
D O I
10.1016/0308-8146(94)90100-7
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A sensitive spectrophotometric method for the determination of aspartame is described. In this method, alpha-chymotrypsin and alcohol oxidase were immobilized on highly porous Amberlite IRA 938 resin by ionic binding, to allow the enzymes to be used more than once. The method involves the enzymatic conversion of aspartame into formaldehyde and hydrogen peroxide by the immobilized alpha-chymotrypsin and alcohol-oxidase system. Horseradish peroxidase was used for the determination of hydrogen peroxide by the formation of a chromophore with 3-methyl-2-benzo-thiazolinone hydrazone (MBTH) and N,N-dimethylaniline (DMA). The absorbances of the sample solutions were measured at 595 nm against a blank. The calibration graph was linear in the range 0.1-1.0 mg ml(-1) of aspartame.
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页码:95 / 97
页数:3
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