MOLECULAR EPIDEMIOLOGY OF XANTHOMONAS-MALTOPHILIA COLONIZATION AND INFECTION IN THE HOSPITAL ENVIRONMENT

被引:85
作者
LAING, FPY
RAMOTAR, K
READ, RR
ALFIERI, N
KUREISHI, A
HENDERSON, EA
LOUIE, TJ
机构
[1] UNIV CALGARY, FAC MED, CALGARY, AB T2N 4N1, CANADA
[2] CALGARY GEN HOSP, CALGARY, AB T2E 0A1, CANADA
[3] CALGARY DIST HOSP, CALGARY, AB T2S 1S69, CANADA
[4] FOOTHILLS PROV GEN HOSP, CALGARY, AB T2N 1N4, CANADA
关键词
D O I
10.1128/JCM.33.3.513-518.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Between April 1992 and December 1993, 80 Xanthomonas maltophilia isolates were collected from 63 patients in three acute-care hospitals in Calgary, Alberta, Canada. On the basis of Centers for Disease Control and Prevention definitions, 48 patients had nosocomial and 15 had community-acquired X. maltophilia. Thirty-eight of the patients were colonized and 25 were infected. Sixty-four percent of patients who acquired X. maltophilia in the intensive care unit (ICU) became infected, whereas 32% of patients in a non-ICU setting became infected. ICU patients tended to be hospitalized for a shorter period of time than non-ICU patients before the onset of X. maltophilia infection. Regardless of being colonized or infected, all patients had debilitating conditions, with respiratory disease being the most common underlying illness (35%). Forty-two patients (88%) with hospital-acquired X. maltophilia received prior antibiotic therapy which included gentamicin, tobramycin, ceftazidime; piperacillin, and imipenem. Agar dilution MICs showed that patient isolates were resistant to these antimicrobial agents that patients had received. Pulsed-field gel electrophoresis of SpeI-digested genomic DNA revealed that six epidemiologically linked patient isolates from the ICU of one acute-care hospital had identical DNA profiles. In contrast, isolates from patients from the other two hospitals had unique genotype profiles (n = 57) regardless of the presence or absence of an epidemiologic association. In these patients there was genetic: evidence against the acquisition of a resident hospital clone. These results indicate that pulsed-field gel electrophoresis can resolve genotypically distinct Strains of X. maltophilia and, consequently, is a useful tool for evaluating nosocomial infections caused by X. maltophilia.
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页码:513 / 518
页数:6
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