PURIFICATION OF PROCOLLAGEN TYPE-II BY COVALENT CHROMATOGRAPHY WITH ACTIVATED THIOL-SEPHAROSE 4B

被引：15

作者：

ANGERMANN, K

BARRACH, HJ

机构：

[1] Institut für Toxikologie, Embryonal-Pharmakologie der Freien Universität Berlin, 1000 Berlin 33

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90356-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Procollagen type II was isolated from Swarm chondrosarcoma of rat by NaCl-extraction and DEAE-cellulose chromatography and further purified from remaining type II collagen using activated thiol-Sepharose 4B for covalent chromatography. The purity of the separated collagen fractions was tested by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results demonstrate that this method is a useful tool for separating procollagen from other collagenous material, which does not contain thiol groups. © 1979.

引用

页码：253 / 258

页数：6

共 26 条

[1] EVIDENCE FOR PROCOLLAGEN, A BIOSYNTHETIC PRECURSOR OF COLLAGEN (ZYMOGEN/PEPSIN DIGESTION/CHROMATOGRAPHY/GEL ELECTROPHORESIS/ISOTOPIC LABELING)
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[2] COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX
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[9] DISULFIDE-BONDED NATURE OF PROCOLLAGEN AND ROLE OF EXTENSION PEPTIDES IN ASSEMBLY OF MOLECULE
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[10] SYNTHESIS AND SECRETION OF CARTILAGE PROCOLLAGEN
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[J]. BIOCHEMICAL JOURNAL, 1975, 148 (01) : 129 - 138

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