INTRACELLULAR PH, CYTOSOLIC CALCIUM-CONCENTRATION AND ELECTRICAL-ACTIVITY IN RINM5F INSULINOMA CELLS

被引：10

作者：

BEST, L ^{[1
]}

AMMALA, C ^{[1
]}

RORSMAN, P ^{[1
]}

TOMLINSON, S ^{[1
]}

机构：

[1] GOTHENBURG UNIV,DEPT MED BIOPHYS,S-40044 GOTHENBURG,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1192卷 / 01期

关键词：

BETA CELL; RINM5F CELL; PH; INTRACELLULAR; CYTOSOLIC CALCIUM ION; MEMBRANE POTENTIAL;

D O I：

10.1016/0005-2736(94)90149-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The addition of L-lactate or acetate to RINm5F cells caused a transient intracellular acidification, an increase in [Ca2+](i) and induced electrical activity. The subsequent withdrawal of lactate or acetate resulted in an intracellular alkalinization with no apparent changes in [Ca2+](i) nor electrical activity. Intracellular alkalinization and acidification by application and withdrawal of NH4Cl were both accompanied by transient increases in [Ca2+](i) in the absence of electrical activity. The induction of electrical activity by lactate was associated with the appearence of inward whole cell currents. Changes in intracellular pH may affect [Ca2+](i) though not necessarily by altering plasma membrane potential. The inward currents associated with lactate application may represent an organic anion conductance contributing towards the stimulation of electrical activity by organic acids.

引用

页码：107 / 111

页数：5