MICROBORE LIQUID-CHROMATOGRAPHY WITH DUAL ELECTROCHEMICAL DETECTION FOR THE DETERMINATION OF SEROTONIN AND 5-HYDROXYINDOLEACETIC ACID IN RAT-BRAIN DIALYSATES

被引：26

作者：

SARRE, S

MICHOTTE, Y

MARVIN, CA

EBINGER, G

机构：

[1] VRIJE UNIV BRUSSELS,INST PHARMACEUT,DEPT PHARMACEUT CHEM & DRUG ANAL,LAARBEEKLAAN 103,B-1090 BRUSSELS,BELGIUM

[2] BAS EUROPE,B-2500 LIER,BELGIUM

[3] VRIJE UNIV BRUSSELS HOSP,DEPT NEUROL,B-1090 BRUSSELS,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 582卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(92)80298-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A microbore liquid chromatographic assay with dual electrochemical detection is described for the determination of serotonin and its metabolite 5-hydroxyindoleacetic acid in rat brain dialysates. The concentration of serotonin in these samples is usually in the low nanomolar range (fmol per 20 mul range). To optimize separation and detection, several adaptations were made to the system with respect to the injection valve, flow-rate of the pump, connections between injector, column and detector, and cell volume of the detector. These aspects are discussed, as well as the procedure developed for optimal peak identification of serotonin and correct estimation of 5-hydroxyindoleacetic acid. The assay allows the measurement of basal serotonin release without the use of a re-uptake inhibitor added to the perfusion fluid.

引用

页码：29 / 34

页数：6

共 14 条

[1] EXTRACELLULAR SEROTONIN AND 5-HYDROXYINDOLEACETIC ACID IN HYPOTHALAMUS OF THE UNANESTHETIZED RAT MEASURED BY INVIVO DIALYSIS COUPLED TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - DIALYSATE SEROTONIN REFLECTS NEURONAL RELEASE [J].