THE DETECTION OF CLONAL PROLIFERATION IN GRANULAR LYMPHOCYTE-PROLIFERATIVE DISORDERS OF NATURAL-KILLER-CELL LINEAGE

被引：42

作者：

SHIMODAIRA, S

ISHIDA, F

KOBAYASHI, H

MAHBUB, B

KAWAHA, K

KITANO, K

机构：

[1] OSAKA MED CTR,IZUMI,OSAKA,JAPAN

[2] RES INST MATERNAL & CHILD HLTH,IZUMI,OSAKA,JAPAN

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1995年 / 90卷 / 03期

关键词：

GLPD; CLONALITY; CHROMOSOMAL ANALYSIS; EBV DNA ANALYSIS; TCR GENE REARRANGEMENT;

D O I：

10.1111/j.1365-2141.1995.tb05587.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The clonal proliferation of large granular lymphocytes can be detected in patients with T-cell-lineage granular lymphocyte-proliferative disorders (T-GLPD) by Southern blotting T-cell receptor genes. However, this cannot be applied to patients with natural killer-cell-lineage GLPD (NK-GLPD) as it lacks a clonal marker. We therefore investigated the use of two other diagnostic techniques in evaluating clonal proliferation in Japanese patients with NK-GLPD (n=4) and T-GLPD (n=3) by chromosomal analysis of peripheral blood mononuclear cells (PBMC) stimulated with either interleukin-2 or phytohaemagglutinin, and Epstein-Barr viral (EBV) genomic DNA analysis, Chromosomal analysis revealed abnormal karyotypes in the PBMC of three of four patients with NK-GLPD, whereas EBV analysis showed a monoclonal terminal configuration in the PBMC in the fourth patient. Southern blots revealed rearrangements of the TCR genes in all three patients with T-GLPD but in none of those with NK-GLPD. It is suggested that these methods may be useful in detecting the abnormal proliferation of large granular lymphocytes in NK-GLPD.

引用

页码：578 / 584

页数：7

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