VASOACTIVE INTESTINAL POLYPEPTIDE - SPECIFIC BINDING TO RAT-BRAIN MEMBRANES

被引：199

作者：

TAYLOR, DP ^{[1
]}

PERT, CB ^{[1
]}

机构：

[1] NIMH,BIOL PSYCHIAT BRANCH BIOCHEM & PHARMACOL SECT,BETHESDA,MD 20014

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 02期

关键词：

D O I：

10.1073/pnas.76.2.660

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The binding of radiolabeled vasoactive intestinal polypeptide (VIP) to rat brain membranes was investigated. Specific binding of 125I-labeled VIP was reversible and saturable (B(max)=2.2 pmol/g of wet tissue). Brain membranes exhibited a high affinity for 125I-labeled VIP (K(D)=1 nM) at a single class of noninteracting sites. Binding of 125I-labeled VIP paralleled its immunohistochemical localization, being enriched in cerebral cortex, hippocampus, striatum, and thalamus, with the notable exception of the hypothalamus, which had low levels of binding. The density of sites was greater in synaptosomal fractions relative to mitochondrial or nuclear fractions. Secretin and partial sequences of it and VIP inhibited binding to brain membranes with an order to potency similar to that found in other systems. The findings suggest the existence of a unique new class of brain receptors.

引用

页码：660 / 664

页数：5

共 55 条

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