3,4,5,3',4'-PENTACHLOROBIPHENYL AS A USEFUL INDUCER FOR PURIFICATION OF RAT-LIVER MICROSOMAL CYTOCHROME P448

被引：22

作者：

OZAWA, N

YOSHIHARA, S

KAWANO, K

OKADA, Y

YOSHIMURA, H

机构：

[1] KYUSHU UNIV,FAC PHARMACEUT SCI,DEPT HYGIEN & FORENS CHEM,FUKUOKA 812,JAPAN

[2] KYUSHU UNIV,FAC SCI,DEPT BIOL,FUKUOKA 812,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91999-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An easy purification of rat liver microsomal cytochrome P448 was performed by using 3,4,5,3′,4′-pentachlorobiphenyl as an inducer. The cytochrome P448, a high spin form, was purified to 18.1 nmoles/mg protein with a good yield by ω-aminooctyl Sepharose 4B column chromatography followed by a hydroxyapatite column chromatography. This hemoprotein cross-reacted with antibody to cytochrome P448 from β-naphthoflavone-treated rats, but not with antibody to cytochrome P450 from phenobarbital-treated rats at all. The results of amino acid analyses suggested that this cytochrome P448 is similar to cytochrome P448 of 3-methylcholanthrene-treated rats. © 1979.

引用

页码：1140 / 1147

页数：8

共 30 条

[11] GEL-ELECTROPHORETICALLY HOMOGENEOUS PREPARATION OF CYTOCHROME-P-450 FROM LIVER-MICROSOMES OF PHENOBARBITAL-PRETREATED RABBITS [J].