PREVENTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE EXPRESSION IN ESCHERICHIA-COLI BY A RIBOZYME

被引：114

作者：

SIOUD, M

DRLICA, K

机构：

[1] PUBL HLTH RES INST CITY NEW YORK INC,455 1ST AVE,NEW YORK,NY 10016

[2] NATL HOSP NORWAY,INST IMMUNOL & RHEUMATOL,N-0172 OSLO,NORWAY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 16期

关键词：

INTRACELLULAR; RNASE-III;

D O I：

10.1073/pnas.88.16.7303

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ribozymes are potentially very powerful agents for perturbing intracellular gene expression. However, pilot experiments in eukaryotes have met with mixed success. We now report that a ribozyme designed to cleave the integrase gene of the human immunodeficiency virus (HIV), when transcribed from a plasmid in Escherichia coli, led to destruction of integrase RNA and complete blockage of integrase protein synthesis. These results indicate that ribozymes can be used to study intracellular gene expression in bacteria and that the HIV-1 integrase gene may be a useful target for therapeutic ribozymes.

引用

页码：7303 / 7307

页数：5

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