DIFFERENTIAL PROCESSING OF PROENKEPHALIN-A BY HUMAN PERIPHERAL-BLOOD MONOCYTES AND LYMPHOCYTES-T

被引:47
作者
KUIS, W
VILLIGER, PM
LESER, HG
LOTZ, M
机构
[1] UNIV CALIF SAN DIEGO, DEPT MED, LA JOLLA, CA 92093 USA
[2] SCRIPPS RES INST, DEPT MOLEC & EXPTL MED, LA JOLLA, CA 92037 USA
关键词
ENKEPHALINS; MONOCYTES; LYMPHOCYTES;
D O I
10.1172/JCI115382
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Human peripheral blood mononuclear cells are analyzed for preproenkephalin gen Met-enkephalin immunoreactivity as detected with a specific antiserum is found in the cytoplasm of monocytes but not in T lymphocytes. Secretion of met-enkephalin was analyzed with an RIA that is specific for the met-enkephalin pentapeptide. Unfractionated PBMC spontaneously released 40 pg/ml met-enkephalin and this increased two- to fourfold after stimulation with PHA. Lower levels (< 100 pg/ml) of met-enkephalin were detected in supernatants from purified T cells that were activated with PHA and IL-2. In contrast, stimulation of purified monocytes with LPS or PMA resulted in the release of up to 600 pg/ml of the processed peptide. To examine whether T cells can produce met-enkephalin precursor peptides, T cell conditioned media were treated with trypsin and carboxypeptidase-B, which is known to release met-enkephalin from the propeptide. This increased levels of met-enkephalin to 400 pg/ml, indicating that lymphocytes secrete the propeptide but do not process it to met-enkephalin. The 1.4-kb preproenkephalin mRNA is detected in activated blood mononuclear cells and in purified monocytes and T cells. To determine whether monocytes or lymphocytes express met-enkephalin in vivo, lymphoid tissues were analyzed by immunohistochemistry. In human spleen tissue, positive cells were found in the red pulp but not in the follicles, which is also consistent with met-enkephalin expression in monocytes. In summary, these results show that human peripheral blood mononuclear cells express preproenkephalin mRNA and that monocytes, but not T cells, process the propeptide to met-enkephalin.
引用
收藏
页码:817 / 824
页数:8
相关论文
共 28 条
  • [11] AN ABOUT 50,000-DALTON PROTEIN IN ADRENAL-MEDULLA - A COMMON PRECURSOR OF [MET]ENKEPHALIN AND [LEU]ENKEPHALIN
    LEWIS, RV
    STERN, AS
    KIMURA, S
    ROSSIER, J
    STEIN, S
    UDENFRIEND, S
    [J]. SCIENCE, 1980, 208 (4451) : 1459 - 1461
  • [12] IMMUNOREACTIVE BETA-ENDORPHIN IN A SUBPOPULATION OF MOUSE SPLEEN MACROPHAGES
    LOLAIT, SJ
    LIM, ATW
    TOH, BH
    FUNDER, JW
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1984, 73 (01) : 277 - 280
  • [13] PROOPIOMELANOCORTIN MESSENGER-RIBONUCLEIC-ACID AND POSTTRANSLATIONAL PROCESSING OF BETA-ENDORPHIN IN SPLEEN MACROPHAGES
    LOLAIT, SJ
    CLEMENTS, JA
    MARKWICK, AJ
    CHENG, C
    MCNALLY, M
    SMITH, AI
    FUNDER, JW
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1986, 77 (06) : 1776 - 1779
  • [14] PREPROENKEPHALIN MESSENGER-RNA IN T-CELLS, MACROPHAGES, AND MAST-CELLS
    MARTIN, J
    PRYSTOWSKY, MB
    ANGELETTI, RH
    [J]. JOURNAL OF NEUROSCIENCE RESEARCH, 1987, 18 (01) : 82 - 87
  • [15] NEUROPEPTIDES - CONDUCTORS OF THE IMMUNE ORCHESTRA
    MORLEY, JE
    KAY, NE
    SOLOMON, GF
    PLOTNIKOFF, NP
    [J]. LIFE SCIENCES, 1987, 41 (05) : 527 - 544
  • [16] SPECIFIC ENZYMATIC AMPLIFICATION OF DNA INVITRO - THE POLYMERASE CHAIN-REACTION
    MULLIS, K
    FALOONA, F
    SCHARF, S
    SAIKI, R
    HORN, G
    ERLICH, H
    [J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1986, 51 : 263 - 273
  • [17] IMMUNOREGULATORY EFFECTS OF ALPHA-ENDORPHIN, BETA-ENDORPHIN, METHIONINE-ENKEPHALIN, AND ADRENOCORTICOTROPIC HORMONE ON ANTI-TETANUS TOXOID ANTIBODY-SYNTHESIS BY HUMAN-LYMPHOCYTES
    MUNN, NA
    LUM, LG
    [J]. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1989, 52 (03): : 376 - 385
  • [18] PLASMA NATIVE AND PEPTIDASE-DERIVABLE MET-ENKEPHALIN RESPONSES TO RESTRAINT STRESS IN RATS - ADAPTATION TO REPEATED RESTRAINT
    PIERZCHALA, K
    VANLOON, GR
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1990, 85 (03) : 861 - 873
  • [19] ROSEN H, 1989, J IMMUNOL, V143, P3703
  • [20] NONOPIATE ACTIVE PROENKEPHALIN-DERIVED PEPTIDES ARE SECRETED BY T-HELPER CELLS
    ROTH, KA
    LORENZ, RG
    UNANUE, RA
    WEAVER, CT
    [J]. FASEB JOURNAL, 1989, 3 (12) : 2401 - 2407