ISOLATION AND PURIFICATION OF AMYLOGLUCOSIDASE FROM HALOBACTERIUM-SODOMENSE

被引：16

作者：

CHAGA, G ^{[1
]}

PORATH, J ^{[1
]}

ILLENI, T ^{[1
]}

机构：

[1] UNIV UPPSALA,BMC,PILOT PLANT,S-75123 UPPSALA,SWEDEN

来源：

BIOMEDICAL CHROMATOGRAPHY | 1993年 / 7卷 / 05期

关键词：

D O I：

10.1002/bmc.1130070504

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Amyloglucosidase from Halobacterium sodomense was purified by a combination of hydrophobic interaction chromatography and immobilized metal ion affinity chromatography at analytical and preparative scale with 75% recovery. The enzyme was found to be a dimer of two different subunits with molecular weights of 72,000 and 82,000 D, respectively, combining in a 175,000 D native protein. The specific activity, K(M), and amino acid composition of the enzyme was determined.

引用

收藏

页码：256 / 261

页数：6

相关论文

共 17 条

[1] PURIFICATION OF COMMERCIAL HUMAN-ALBUMIN ON IMMOBILIZED IDA-NI2+ [J].

ANDERSSON, L ;

SULKOWSKI, E ;

PORATH, J .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 421 (01) :141-146

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] PURIFICATION AND DETERMINATION OF THE BINDING-SITE OF LACTATE-DEHYDROGENASE FROM CHICKEN BREAST MUSCLE ON IMMOBILIZED FERRIC IONS [J].

CHAGA, G ;

ANDERSSON, L ;

PORATH, J .

JOURNAL OF CHROMATOGRAPHY, 1992, 627 (1-2) :163-172

[4]

CHAGA G, 1989, BIOTECHNOL APPL BIOC, V11, P424

[5] USE OF IMMOBILIZED METAL-IONS AS A NEGATIVE ADSORBENT FOR PURIFICATION OF ENZYMES - APPLICATION TO PHOSPHOGLYCERATE MUTASE FROM CHICKEN MUSCLE EXTRACT AND HORSERADISH-PEROXIDASE [J].

CHAGA, G ;

ANDERSSON, L ;

ERSSON, B ;

BERG, M .

BIOMEDICAL CHROMATOGRAPHY, 1992, 6 (04) :172-176

[6] ISOLATION AND CHARACTERIZATION OF CATALASE FROM PENICILLIUM-CHRYSOGENUM [J].

CHAGA, GS ;

MEDIN, AS ;

CHAGA, SG ;

PORATH, JO .

JOURNAL OF CHROMATOGRAPHY, 1992, 604 (01) :177-183

[7]

DEMOT R, 1987, EUR J BIOCHEM, V164, P643

[8] PURIFICATION AND CHARACTERIZATION OF A MINOR GLUCOAMYLASE FROM ASPERGILLUS-SAITOI [J].

INOKUCHI, N ;

TAKAHASHI, T ;

IRIE, M .

JOURNAL OF BIOCHEMISTRY, 1981, 90 (04) :1055-1067

[9] THERMOPHILIC ALANINE DEHYDROGENASE FROM HALOBACTERIUM-SALINARIUM [J].

KERADJOPOULOS, D ;

WULFF, K .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1974, 52 (11) :1033-1037

[10] PURIFICATION AND PROPERTIES OF AN EXTRACELLULAR GLUCOAMYLASE FROM A DIASTATIC STRAIN OF SACCHAROMYCES-CEREVISIAE [J].

KLEINMAN, MJ ;

WILKINSON, AE ;

WRIGHT, IP ;

EVANS, IH ;

BEVAN, EA .

BIOCHEMICAL JOURNAL, 1988, 249 (01) :163-170