FRAGMENTATION CHARACTERISTIC OF GLUTATHIONE CONJUGATES ACTIVATED BY HIGH-ENERGY COLLISIONS

被引：28

作者：

MURPHY, CM

FENSELAU, C

GUTIERREZ, PL

机构：

[1] UNIV MARYLAND, DEPT CHEM & BIOCHEM, 5401 WILKENS AVE, CATONSVILLE, MD 21228 USA

[2] UNIV MARYLAND, CTR CANC, BALTIMORE, MD 21201 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1992年 / 3卷 / 08期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/1044-0305(92)80004-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Product ion spectra of fifteen monoglutathione and diglutathione conjugates have been measured using activation by 6000-eV collisions with helium in the third field-free region of a four-sector tandem mass spectrometer of EBEB configuration. Fragmentation patterns in the cation spectra have been analyzed for decompositions of the glutathione moiety that would permit recognition of an unknown as a glutathione conjugate. Five spectra from an earlier study of high-energy collisional activation on a BEEB four-sector instrument have also been included in this analysis. A suite of appropriate ions was found to occur consistently, including ions of m/z 307 comprising the glutathione tripeptide and the complementary ion [MH - 307]+ or the ion radical [MH - 306]+.

引用

页码：815 / 822

页数：8

共 30 条

[1] THE USE OF MASS-SPECTROMETRY IN THE STUDY OF CHEMICALLY-REACTIVE DRUG METABOLITES - APPLICATION OF MS/MS AND LC/MS TO THE ANALYSIS OF GLUTATHIONE-LINKED AND RELATED S-LINKED CONJUGATES OF N-METHYLFORMAMIDE [J].