CHONDROITIN SULFATE IN THE EXTRACELLULAR-MATRIX OF THE MEDIAL AND LATERAL SUPERIOR OLIVARY NUCLEI IN THE DOG

被引:17
作者
ATOJI, Y
SUZUKI, Y
机构
[1] Laboratory of Veterinary Anatomy, Faculty of Agriculture, Gifu University, Gifu
关键词
EXTRACELLULAR MATRIX; CHONDROITIN SULFATE; LATERAL SUPERIOR OLIVARY NUCLEUS; MEDIAL SUPERIOR OLIVARY NUCLEUS; DOG; IMMUNOCYTOCHEMISTRY;
D O I
10.1016/0006-8993(92)91220-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Chondroitin sulfate was examined in the extracellular matrix of the canine medial and lateral superior olivary nuclei by light and electron microscopic immunocytochemistry. The extracellular matrix around the large neurons was intensely stained with a monoclonal antibody recognizing D-glucuronic acid 2-sulfate --> N-acetylgalactosamine 6-sulfate (D-unit) and this staining degree was remarkably reduced after chondroitinase ABC digestion. Neuronal cytoplasm, glial cells or capillaries in these nuclei were not stained with the monoclonal antibody. The results indicate the presence of disaccharide residue Of D-glucuronic acid 2-sulfate --> N-acetylgalactosamine 6-sulfate in the chondroitin sulfate proteoglycan of the extracellular matrix.
引用
收藏
页码:287 / 290
页数:4
相关论文
共 22 条
[21]  
YAMAGATA M, 1987, J BIOL CHEM, V262, P4146
[22]   CHARACTERIZATION OF AN ACTIVITY-DEPENDENT, NEURONAL SURFACE PROTEOGLYCAN IDENTIFIED WITH MONOCLONAL-ANTIBODY CAT-301 [J].
ZAREMBA, S ;
GUIMARAES, A ;
KALB, RG ;
HOCKFIELD, S .
NEURON, 1989, 2 (03) :1207-1219