TRANSCRIPTIONAL ACTIVITY OF THE CD45 GENE PROMOTER IN RETROVIRAL VECTOR CONSTRUCTS

被引:5
作者
ANSON, DS
OCCHIODORO, T
机构
[1] Lysosomal Diseases Research Unit, Department of Chemical Pathology, Centre for Medical Genetics, SA 5006, 72 King William Rd., N. Adelaide
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1219卷 / 01期
关键词
GENE THERAPY; TISSUE SPECIFIC EXPRESSION;
D O I
10.1016/0167-4781(94)90249-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Both specific and housekeeping transcriptional control elements have been successfully used to generate retroviral vectors that express in a stable tissue specific manner. However, no such vectors have been developed specifically for expression in haematopoietic cells. This paper describes the construction of retroviral vectors incorporating sequences from the promoter region of the murine CD45 (leucocyte common antigen) gene and using the human alpha-L-iduronidase cDNA as a reporter gene. These vectors were tested for expression of the alpha-L-iduronidase gene in skin fibroblasts and a lymphoblastiod cell line which is permissive for CD45 expression, derived from patients with mucopolysaccharidosis type I(MPS I, alpha-L-iduronidase deficiency). The results obtained demonstrate that, in the context of a retroviral construct, the activity of the CD45 gene promoter in lymphocytes and fibroblasts parallels the activity of the retroviral long terminal repeat (LTR). These results suggest that additional transcriptional control elements from the CD45 gene, or other haematopoietic specific genes, will be necessary for the construction of a haematopoietic specific vector.
引用
收藏
页码:81 / 88
页数:8
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