MEASUREMENT OF TISSUE SULFHYDRYLS AND DISULFIDES IN TISSUE PROTEIN AND NONPROTEIN FRACTIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ELECTROCHEMICAL DETECTION

被引：30

作者：

DUPUY, D ^{[1
]}

SZABO, S ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1987年 / 10卷 / 01期

关键词：

BIOCHEMISTRY - BIOLOGICAL MATERIALS - Chromatographic Analysis - ELECTROCHEMISTRY - PROTEINS - Chromatographic Analysis;

D O I：

10.1080/01483918708074195

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific, sensitive and quantitative method for measuring tissue sulfhydryl (SH) and disulfide levels in nonprotein, protein, and protein-bound fraction has been developed by using HPLC with electrochemical detection. Protein and nonprotein fractions are separated through perchloric acid precipitation. The protein fraction is divided into two aliquots: one which undergoes protein hydrolysis in HCl and the other which undergoes sodium borohydride reduction. The nonprotein, protein and protein-bound fractions generated are then separated by HPLC and the various sulfhydryls (e. g. , cysteine, glutathione) and disulfides (e. g. , cystine, glutathione disulfide) are measured directly by a dual gold mercury electrode thin layer electrochemical cell.

引用

页码：107 / 119

页数：13