CHARACTERIZATION OF YEAST-EXPRESSED BETA-ACTINS, SITE-SPECIFICALLY MUTATED AT THE TUMOR-RELATED RESIDUE GLY245

被引：17

作者：

ASPENSTROM, P

ENGKVIST, H

LINDBERG, U

KARLSSON, R

机构：

[1] UNIV UPPSALA,CTR BIOMED,DEPT DEV BIOL,BOX 587,S-75123 UPPSALA,SWEDEN

[2] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT ZOOL CELLBIOL,WGI,S-10691 STOCKHOLM,SWEDEN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 207卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1992.tb17052.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tumorigenic cell line HUT14 expresses a beta-actin carrying a mutation at position 245. In this study, two mutant beta-actins with amino acid changes at position 245 replacing the wild-type glycine by an aspartic acid and a lysine residue, respectively, were produced in the yeast Saccharomyces cerevisiae, purified to homogeneity and characterized with respect to polymerization behaviour and interaction with myosin. The major functional effect of these mutations appears to be an impaired polymerization, while the interaction with myosin seems less influenced. In addition, the results also suggest the presence of a Ca2+-binding site in the region of residue 245 in actin.

引用

页码：315 / 320

页数：6

共 61 条

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