VISUALIZATION OF PROTEIN-TRANSPORT ALONG THE SECRETORY PATHWAY USING GREEN FLUORESCENT PROTEIN

被引：109

作者：

KAETHER, C ^{[1
]}

GERDES, HH ^{[1
]}

机构：

[1] UNIV HEIDELBERG,INST NEUROBIOL,D-69120 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1995年 / 369卷 / 2-3期

关键词：

GREEN FLUORESCENT PROTEIN; GFP; CHROMOGRANIN B; SECRETORY PATHWAY; INTERMEDIATE COMPARTMENT; TRANS-GOLGI NETWORK;

D O I：

10.1016/0014-5793(95)00765-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was observed when biosynthetic protein transport was arrested at the intermediate compartment or the tr trans-Golgi network by temperature blocks (15 degrees C and 20 degrees C, respectively). Reversal of the temperature blocks allowed the visualization of secretion of fluorescent GFP and offers the possibility to analyse transport in the secretory pathway in living cells.

引用

页码：267 / 271

页数：5

共 18 条

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