A NEW U2 RNA SECONDARY STRUCTURE PROVIDED BY PHYLOGENETIC ANALYSIS OF TRYPANOSOMATID U2 RNAS

被引：38

作者：

HARTSHORNE, T ^{[1
]}

AGABIAN, N ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH PHARM,INTERCAMPUS PROGRAM MOLEC PARASITOL,SAN FRANCISCO,CA 94143

来源：

GENES & DEVELOPMENT | 1990年 / 4卷 / 12A期

关键词：

secondary structure; trypanosomatids; U2; RNA;

D O I：

10.1101/gad.4.12a.2121

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A new model of U2 RNA secondary structure has been developed by comparing U2 RNA sequences from distantly related trypanosomatids, which process their RNAs by trans-splicing, and U2 RNAs from cis-splicing organisms. The trypanosomatid U2 RNA retains structural similarity in important functional domains of cis-splicing U2 RNAs yet differs from previous consensus models in that only two helices, rather than three, can form in the stem-loop II region. This alteration eliminates the capacity for pseudoknot formation and produces a single-stranded region 3' to stem-loop II, which may be accessible for snRNP protein binding. Trypanosomatid U2 RNAs differ in the putative branchpoint recognition sequences, which completely diverge from the conserved GUAGUA consensus of cis-splicing organisms.

引用

页码：2121 / 2131

页数：11

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