ABNORMAL CHLORIDE CONDUCTANCE IN MULTIDRUG RESISTANT HL60/AR CELLS

被引：17

作者：

GOLLAPUDI, S

MCDONALD, T

GARDNER, P

KANG, N

GUPTA, S

机构：

[1] UNIV CALIF IRVINE,DIV BASIC & CLIN IMMUNOL,ROOM C-264A,IRVINE,CA 92717

[2] STANFORD UNIV,FALK CARDIOVASC RES CTR,SCH MED,DEPT MED,STANFORD,CA 94305

来源：

CANCER LETTERS | 1992年 / 66卷 / 01期

关键词：

MULTIDRUG RESISTANCE; INTRACELLULAR PH; CHLORIDE CURRENT;

D O I：

10.1016/0304-3835(92)90284-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Chloride channel currents were measured in drug sensitive parental HL60 and multidrug resistant (MDR) subline HL60/AR cells, using a whole cell patch-clamp technique. In addition, the in vitro effects of 4,4'diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), a Cl- channel blocker, on intracellular accumulation and sensitivity to daunorubicin and intracellular pH (pH(i)) in HL60 cells were examined. Baseline DIDS blockable Cl- currents were consistently lower in HL60/AR cells (0.9 pA/pF) as compared to HL60 cells (7.0 pA/pF). Similarly cAMP-activated Cl- currents were minimal in HL60/AR cells (0.2 pA/pF) as compared to HL60 cells (8 pA/pF). In vitro treatment of drug sensitive HL60 cells with DIDS resulted in concentration-dependent decreased accumulation and increased resistance to daunorubicin and decreased pH(i). These data show that altered Cl- permeability is associated with MDR and suggest that Cl- channels may play a role in MDR.

引用

页码：83 / 89

页数：7