IMMUNOHISTOCHEMICAL DEMONSTRATION OF ETHANOL-INDUCIBLE P450 2E1 IN RAT-BRAIN

被引:30
作者
SOHDA, T
SHIMIZU, M
KAMIMURA, S
OKUMURA, M
机构
来源
ALCOHOL AND ALCOHOLISM | 1993年 / 28卷
关键词
D O I
10.1093/alcalc/28.Supplement_1B.69
中图分类号
R194 [卫生标准、卫生检查、医药管理];
学科分类号
摘要
The microsomal ethanol-oxidizing system (MEOS) is a P450-dependent pathway for ethanol oxidation in hepatic microsomes. The bulk of MEOS activity is catalyzed by P450 2E1 (an ethanol-inducible isozyme of P450) in animal livers treated with ethanol. Rat brains also metabolize certain drugs, and it is theorized that a mechanism for drug metabolism exists within the brain which acts on P450. We compared immunohistochemical localization of P450 2E1 between ethanol-treated rats and pair-fed control rats. In control rats, immunoreactive P450 2E1 was detected in minute amounts in both basal ganglia and cerebellar cortices. After ethanol treatment, the content of P450 2E1 increased in the basal ganglia, and the enzyme was also induced in the cerebellar cortices, substantia nigra and hippocampus. We speculate that the rat brain metabolizes ethanol by P450 2E1.
引用
收藏
页码:69 / 75
页数:7
相关论文
共 16 条
[1]   PURIFICATION OF NADPH - CYTOCHROME-C (CYTOCHROME-P-450) REDUCTASE FROM HAMSTER LIVER-MICROSOMES BY DETERGENT EXTRACTION AND AFFINITY-CHROMATOGRAPHY [J].
ARDIES, CM ;
LASKER, JM ;
BLOSWICK, BP ;
LIEBER, CS .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :39-46
[2]   ROLE OF CYTOCHROME-P-450 2E1 IN ETHANOL-DEPENDENT, CARBON TETRACHLORIDE-DEPENDENT AND IRON-DEPENDENT MICROSOMAL LIPID-PEROXIDATION [J].
CASTILLO, T ;
KOOP, DR ;
KAMIMURA, S ;
TRIADAFILOPOULOS, G ;
TSUKAMOTO, H .
HEPATOLOGY, 1992, 16 (04) :992-996
[3]  
FARINATI F, 1985, DRUG METAB DISPOS, V13, P210
[4]   CATECHOL ESTROGEN FORMATION IN CENTRAL NERVOUS-SYSTEM OF RAT [J].
FISHMAN, J ;
NORTON, B .
ENDOCRINOLOGY, 1975, 96 (04) :1054-1059
[5]   N-DEMETHYLATION OF MORPHINE IN RAT-BRAIN IS LOCALIZED IN SITES WITH HIGH OPIATE RECEPTOR CONTENT [J].
FISHMAN, J ;
HAHN, EF ;
NORTON, BI .
NATURE, 1976, 261 (5555) :64-65
[6]  
GARRO AJ, 1981, CANCER RES, V41, P120
[7]   IMMUNOHISTOCHEMICAL LOCALIZATION OF CYTOCHROME-P-450 IN RAT-BRAIN [J].
KAPITULNIK, J ;
GELBOIN, HV ;
GUENGERICH, FP ;
JACOBOWITZ, DM .
NEUROSCIENCE, 1987, 20 (03) :829-833
[8]  
LIEBER CS, 1989, ALCOHOL ALCOHOLISM, V24, P197
[9]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[10]  
OMURA T, 1964, J BIOL CHEM, V239, P2370