Reaction kinetics for the hydrolysis of tributyrin using an immobilized bacterial lipase

被引：6

作者：

Warmuth, W. ^{[1
]}

Wenzig, E. ^{[1
]}

Mersmann, A. ^{[1
]}

机构：

[1] Tech Univ Munich, Dept Chem Engn B, D-80333 Munich, Germany

来源：

CHEMICAL ENGINEERING SCIENCE | 1994年 / 49卷 / 24B期

关键词：

D O I：

10.1016/0009-2509(94)00375-0

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The kinetics of the hydrolysis of tributyrin to free fatty acid and glycerol using bacterial lipase from Staphylococcus carnosus (pLipMut2) was investigated in a packed bed recycle reactor. The effect of reaction temperature, diameter of the support particles, diameter of the oil droplets and recycle rate on the course of reaction were investigated and the results were used to select experimental conditions so the reaction rate would not be limited by mass transfer. A model for predicting the rate of reaction was developed from elementary steps assuming the surface reaction at the active site of the enzyme to be rate limiting. The proposed model describes the influence of substrate concentration on the rate of reaction as well as the inhibitory effect of free fatty acids and glycerol.

引用

页码：5087 / 5095

页数：9

共 21 条

[1] HYDROLYSIS OF TRIGLYCERIDE BY SOLID-PHASE LIPOLYTIC ENZYMES OF RHIZOPUS-ARRHIZUS IN CONTINUOUS REACTOR SYSTEMS [J].

BELL, G ;

TODD, JR ;

BLAIN, JA ;

PATTERSON, JDE ;

SHAW, CEL .

BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (08) :1703-1719

[2] ETUDE CINETIQUE DE LACTION DE LA LIPASE PANCREATIQUE SUR DES TRIGLYCERIDES EN EMULSION ESSAI DUNE ENZYMOLOGIE EN MILIEU HETEROGENE [J].

BENZONANA, G ;

DESNUELLE, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 105 (01) :121-+

[3] STAINING METHOD FOR DETERMINATION OF THE PENETRATION OF IMMOBILIZED ENZYME INTO A POROUS SUPPORT [J].

CARLEYSMITH, SW ;

EAMES, MBL ;

LILLY, MD .

BIOTECHNOLOGY AND BIOENGINEERING, 1980, 22 (05) :957-967

[4]

CHA S, 1968, J BIOL CHEM, V243, P820

[5] REACTION ENGINEERING [J].

EMIG, G ;

HOFMANN, H .

CHEMIE INGENIEUR TECHNIK, 1975, 47 (17) :717-720

[6] USE OF CANDIDA-RUGOSA LIPASE IMMOBILIZED IN A SPIRAL WOUND MEMBRANE REACTOR FOR THE HYDROLYSIS OF MILKFAT [J].

GARCIA, HS ;

MALCATA, FX ;

HILL, CG ;

AMUNDSON, CH .

ENZYME AND MICROBIAL TECHNOLOGY, 1992, 14 (07) :535-545

[7] LIPASE KINETICS - HYDROLYSIS OF TRIACETIN BY LIPASE FROM CANDIDA-CYLINDRACEA IN A HOLLOW-FIBER MEMBRANE REACTOR [J].

GUIT, RPM ;

KLOOSTERMAN, M ;

MEINDERSMA, GW ;

MAYER, M ;

MEIJER, EM .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (07) :727-732

[8] BIOREACTOR FOR ENZYMATIC-REACTION OF FAT AND FATTY-ACID DERIVATIVES .3. CONTINUOUS HYDROLYSIS OF OLIVE OIL BY LIPASE IN MICROPOROUS HYDROPHOBIC MEMBRANE BIOREACTOR [J].

HOQ, MM ;

YAMANE, T ;

SHIMIZU, S ;

FUNADA, T ;

ISHIDA, S .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1985, 62 (06) :1016-1021

[9]

JUNG HJ, 1992, CHEM ENG TECHNOL, V15, P341

[10] ADSORPTION BEHAVIOR OF LIPASE FROM STAPHYLOCOCCUS-CARNOSUS ON A HYDROPHOBIC ADSORBENT [J].

KERZEL, P ;

MERSMANN, A .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 584 (01) :109-113

← 1 2 3 →