ENZYME-MICROENVIRONMENT DYNAMIC INTERACTIONS IN MICROSTRUCTURED MEDIA

被引：9

作者：

CHOPINEAU, J ^{[1
]}

OLLIVON, M ^{[1
]}

THOMAS, D ^{[1
]}

LEGOY, MD ^{[1
]}

机构：

[1] UNIV PARIS SUD,CNRS,URA 1218,EQUIPE PHYSICOCHIM SYST POLYPHASES,F-92296 CHATENAY MALABRY,FRANCE

来源：

PURE AND APPLIED CHEMISTRY | 1992年 / 64卷 / 11期

关键词：

D O I：

10.1351/pac199264111757

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new approach for the study of an enzyme's relationship with its own reaction medium is described. The microheterogeneous (micellar and liquid crystalline) media octyl-beta-D-glucoside/water (or glucose in water)/octanol plus the enzyme beta-D-Glucosidase were used to study enzyme-microenvironment dynamic interactions. Beta-D-Glucosidase hydrolyses octyl-beta-D-glucoside to form glucose and octanol. So doing, it could cause a transition from a one-phase domain of the diagram to a polyphasic system. Starting from each homogeneous region of the phase diagram, the enzyme, due to its catalytic activity, was responsible for different phase changes. The physicochemical properties of the medium changed in proportion to the progress of the enzymatic reaction. The modifications of the microenvironment were correlated with kinetic patterns specific for each phase exploration. Enzymatic reaction rate modifications occuring during the transitions were function of the different microstructured phases in presence but they were also dependent on local concentration and disponibility of octyl-beta-D-glucoside monomer.

引用

页码：1757 / 1763

页数：7