ANALYSIS OF ALL STRATUM-CORNEUM LIPIDS BY AUTOMATED MULTIPLE DEVELOPMENT HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

被引：46

作者：

BONTE, F

PINGUET, P

CHEVALIER, JM

MEYBECK, A

机构：

[1] LVMH Recherche, 92752 Nanterre

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00480-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An optimized gradient enabling the separation of all stratum corneum lipids by automated multiple development on HPTLC plates is presented. An initial isocratic step separates sebum lipids. This is followed by a 25-step development using a gradient with a polarity range of methanol-water to hexane. Application to in-vivo extracted and isolated stratum corneum lipids demonstrates the possible quantification of the lipid classes with a ''one-experiment'' separation.

引用

页码：311 / 316

页数：6

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