AN HPLC POSTCOLUMN REACTION SYSTEM FOR PHOSPHORUS-SPECIFIC DETECTION IN THE COMPLETE SEPARATION OF INOSITOL PHOSPHATE CONGENERS IN AQUEOUS SAMPLES

被引：19

作者：

CLARKIN, CM ^{[1
]}

MINEAR, RA ^{[1
]}

KIM, S ^{[1
]}

ELWOOD, JW ^{[1
]}

机构：

[1] UNIV ILLINOIS,INST ENVIRONM STUDIES,1101 W PEABODY DR,URBANA,IL 61801

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 1992年 / 26卷 / 01期

关键词：

D O I：

10.1021/es00025a025

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A method for the separation of all the inositol phosphate congeners has been developed and demonstrated. Phosphorus-specific detection is achieved by postcolumn illumination by high-intensity ultraviolet radiation with resultant formation of reduced phosphomolybdate by flow injection of an ascorbic acid/molybdate reagent and spectrophotometric detection. Separations are achieved with a complex gradient between 0.1 and 0.5 N NaCl and constant concentration of Na4EDTA (0.5 mM). Submilligram per liter levels of phosphorus can be detected with 50-250-mu-L injections. Preliminary results with field samples are presented along with a proposed sample concentration procedure necessary to obtain sufficient phosphorus levels.

引用

页码：199 / 204

页数：6

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