A NEWLY ISOLATED BACILLUS STEAROTHERMOPHILUS K1041 AND ITS TRANSFORMATION BY ELECTROPORATION

被引：32

作者：

Narumi, Issay ^{[1
]}

Sawakami, Kazumi ^{[1
]}

Nakamoto, Shinya ^{[2
]}

Nakayama, Noriyuki ^{[2
]}

Yanagisawa, Tadashi ^{[1
]}

Takahashi, Nobutaka ^{[3
]}

Kihara, Hiroshi ^{[4
]}

机构：

[1] Utsunomiya Univ, Dept Agr Chem, Utsunomiya, Tochigi 321, Japan

[2] NEC Corp Ltd, Miyamae Ku, Kawasaki, Kanagawa 216, Japan

[3] Inst Phys & Chem Res, Wako, Saitama 35101, Japan

[4] Jichi Med Sch, Sch Nursing, Minami Kawachi, Tochigi 32904, Japan

来源：

BIOTECHNOLOGY TECHNIQUES | 1992年 / 6卷 / 01期

关键词：

D O I：

10.1007/BF02438695

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimal conditions for the plasmid transformation of a newly isolated Bacillus stearothermophilus K1041 by electroporation were investigated. The optimal conditions allowed a transformation efficiency of 5.8 x 10(5) transformants per mu g plasmid pUB110.

引用

页码：83 / 86

页数：4

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