REGULATION OF MG2+ UPTAKE IN ISOLATED RAT MYOCYTES AND HEPATOCYTES BY PROTEIN-KINASE-C

被引：51

作者：

ROMANI, A

MARFELLA, C

SCARPA, A

机构：

[1] Department of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland

来源：

FEBS LETTERS | 1992年 / 296卷 / 02期

关键词：

MG2+ TRANSPORT; PROTEIN KINASE-C; HEPATOCYTE; MYOCYTE;

D O I：

10.1016/0014-5793(92)80364-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A large Mg2+ cell uptake against concentration gradients is stimulated in collagenase-dispersed rat myocytes by carbachol and in hepatocytes by carbachol or vasopressin. The signalling pathway(s) responsible for this stimulation of Mg2+ uptake was investigated by using various activators or inhibitors of protein kinase C (PKC) and by correlating Mg2+ uptake with cell PKC activity and cAMP content. In both cell preparations, the direct stimulation of PKC by diacylglycerol analogs or phorbol esters reproduce the same pattern of Mg2+ uptake as that induced by carbachol or vasopressin. These data indicate that the activation of PKC is responsible for a stimulation of Mg2+ uptake by myocytes or hepatocytes, whereas increase in cAMP in these cells stimulates Mg2+ release.

引用

页码：135 / 140

页数：6

共 42 条

[11]

FABIATO A, 1988, METHOD ENZYMOL, V157, P378

[12] DOWN-REGULATION OF PROTEIN KINASE-C PROTECTS CEREBELLAR GRANULE NEURONS IN PRIMARY CULTURE FROM GLUTAMATE-INDUCED NEURONAL DEATH [J].