ONLINE MONITORING OF HYBRIDOMA CELL-GROWTH USING A LASER TURBIDITY SENSOR

被引：55

作者：

KONSTANTINOV, KB

PAMBAYUN, R

MATANGUIHAN, R

YOSHIDA, T

PERUSICH, CM

HU, WS

机构：

[1] OSAKA UNIV,FAC ENGN,SUITA,OSAKA 565,JAPAN

[2] UNIV MINNESOTA,DEPT CHEM ENGN & MAT SCI,MINNEAPOLIS,MN 55455

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 40卷 / 11期

关键词：

HYBRIDOMA GROWTH; TURBIDITY SENSOR; CELL CONCENTRATION MONITORING; BIOPROCESS CONTROL; GROWTH RATE;

D O I：

10.1002/bit.260401107

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A high-sensitivity turbidity probe was used for on-line monitoring of the cell concentration in batch hybridoma cultivation. Good correlation between off-line cell counts and the linearized sensor signal was found. The quality of the signal was sufficiently high to provide for on-line estimation of the specific growth rate using an efficient filtering procedure. These positive results suggest that such laser turbidity sensors will facilitate development of systems for on-line monitoring and control of animal cell cultivations.

引用

页码：1337 / 1342

页数：6

共 27 条

[1] LARGE-SCALE PRODUCTION OF MONOCLONAL-ANTIBODIES IN SUSPENSION-CULTURE [J].