DETECTION OF FOOT-AND-MOUTH-DISEASE VIRUS IN NASAL SWABS OF ASYMPTOMATIC CATTLE BY RT-PCR WITHIN 24 HOURS

被引：32

作者：

MARQUARDT, O

STRAUB, OC

AHL, R

HAAS, B

机构：

[1] Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tubingen, D-72001

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 53卷 / 2-3期

关键词：

FOOT-AND-MOUTH DISEASE (FMD); POLYMERASE CHAIN REACTION; NASAL SWAB; DIAGNOSIS;

D O I：

10.1016/0166-0934(95)00015-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for extracting RNA from animal-derived materials that provides foot-and-mouth disease viral template suitable for Tth polymerase-dependent synthesis of cDNA and subsequent PCR is described. Viral genomes were detected in less than 24 h. Nasal swabs that can be easily and repeatedly collected, proved suitable for virus detection by PCR, even during the asymptomatic stages of infection.

引用

页码：255 / 261

页数：7