FATE OF MESSENGER-RNA FOLLOWING DISAGGREGATION OF BRAIN POLYSOMES AFTER ADMINISTRATION OF (+)-LYSERGIC ACID DIETHYLAMIDE INVIVO

被引：12

作者：

MAHONY, JB ^{[1
]}

BROWN, IR ^{[1
]}

机构：

[1] UNIV TORONTO,SCARBOROUGH COLL,DEPT ZOOL,W HILL M1C 1A4,ONTARIO,CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 565卷 / 01期

基金：

英国医学研究理事会;

关键词：

(Brain); Lysergic acid diethylamide; mRNA conservation; Polysome disaggregation;

D O I：

10.1016/0005-2787(79)90092-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intravenous injection of (+)-lysergic acid diethylamide into young rabbits induced a transient brain-specific disaggregation of polysomes to monosomes. Investigation of the fate of mRNA revealed that brain poly(A+)mRNA was conserved. In particular, mRNA coding for brain-specific S100 protein was not degraded, nor was it released into free ribonucleoprotein particles. Following the (+)-lysergic acid diethylamide-induced disaggregation of polysomes, mRNA shifted from polysomes and accumulated on monosomes. Formation of a blocked monosome complex, which contained intact mRNA and 40-S plus 60-S ribosomal subunits but lacked nascent peptide chains, suggested that (+)-lysergic acid diethylamide inhibited brain protein synthesis at a specific stage of late initiation or early elongation. © 1979.

引用

页码：161 / 172

页数：12

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