ELECTROPORATION AND PEG DELIVERY OF DNA INTO MAIZE MICROSPORES

被引:8
作者
FENNELL, A [1 ]
HAUPTMANN, R [1 ]
机构
[1] NO ILLINOIS UNIV,CTR PLANT MOLEC BIOL,DE KALB,IL 60115
关键词
MICROSPORE; ELECTROPORATION; TRANSFORMATION; ZEA-MAYS;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The ability to deliver and detect reporter gene activity in maize microspores was tested. Tested expression vectors contained the chloramphenicol acetyl transferase (CAT) gene and one of the following promoter-intron combinations: 1) cauliflower mosaic virus (CaMV 35S), 2) CaMV 35S + maize alcohol dehydrogenase 1 intron 6 (Adh1-I6), 3) maize alcohol dehydrogenase 1 + intron 1 (Adh1-I1), or 4) maize ubiquitin 1 + intron 1 (Ubiq 1-I1) promoter + intron. The expression vectors were delivered into maize microspores using electroporation or polyethylene glycol (PEG). Both methods were effective for delivering free DNA into microspores. Although all four promoters were active in maize protoplasts, only two promoters were active in maize microspores. The CaMV 35S and the Adh1 promoters did not promote gene expression in maize microspore. The CaMV 35S + Adh1-I6 and Ubiq1-I1 promoters produced high levels of CAT activity in maize microspores.
引用
收藏
页码:567 / 570
页数:4
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