DIFFERENTIAL RNA EDITING IN CLOSELY-RELATED INTRONS IN OENOTHERA MITOCHONDRIA

被引：33

作者：

LIPPOK, B ^{[1
]}

BRENNICKE, A ^{[1
]}

WISSINGER, B ^{[1
]}

机构：

[1] INST GENBIOL FORSCH,D-14195 BERLIN,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 243卷 / 01期

关键词：

PLANT MITOCHONDRIA; RNA EDITING SPECIFICITY; INTRON GROUP II FAMILIES;

D O I：

10.1007/BF00283874

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Introns a/b of the nad2 gene and b/c of the nad1 gene in Oenothera mitochondria were found to be closely related. Within a scaffold of conserved sequence regions, a 48 bp sequence element covering intron domain V and flanking nucleotides is identical in both group II introns. The third nucleotide of this element is edited in the nad2, but not in the nad1 intervening sequence. The C to U editing event compensates an nad2-specific nucleotide mismatch in the stem domain IV and thus improves secondary structure stability. This differential editing event indicates that the identical upstream 2 and downstream 45 nucleotides are not sufficient to specify this editing site. Comparison of adjacent exon editing patterns in spliced and unspliced transcripts shows a higher degree of editing in processed sequences, confirming that RNA editing is a posttranscriptional process in plant mitochondria.

引用

页码：39 / 46

页数：8

共 34 条

[31] SPECIES-SPECIFIC RNA EDITING PATTERNS IN THE MITOCHONDRIAL RPS13 TRANSCRIPTS OF OENOTHERA AND DAUCUS [J].