DENSITY ANALYSIS OF HEPATITIS-C VIRUS PARTICLE-POPULATION IN THE CIRCULATION OF INFECTED HOSTS - IMPLICATIONS FOR VIRUS NEUTRALIZATION OR PERSISTENCE

被引：62

作者：

KANTO, T ^{[1
]}

HAYASHI, N ^{[1
]}

TAKEHARA, T ^{[1
]}

HAGIWARA, H ^{[1
]}

MITA, E ^{[1
]}

NAITO, M ^{[1
]}

KASAHARA, A ^{[1
]}

FUSAMOTO, H ^{[1
]}

KAMADA, T ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT MED 1,SUITA,OSAKA 565,JAPAN

来源：

JOURNAL OF HEPATOLOGY | 1995年 / 22卷 / 04期

关键词：

ACUTE HEPATITIS C; BUOYANT DENSITY; CHRONIC HEPATITIS C; HEPATITIS C VIRUS; IMMUNOPRECIPITATION;

D O I：

10.1016/0168-8278(95)80107-3

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Hepatitis C virus has a low buoyant density in sucrose, but high-density particles are often observed in hepatitis C virus infection. To investigate the characteristics of circulating hepatitis C virus particles and their association with liver disease progression, we examined sera from two histologically normal hepatitis C virus carriers, 20 chronic hepatitis patients and five acute hepatitis C patients, The supernatants obtained after immunoprecipitation with anti-immunoglobulins antibody were subjected to sucrose equilibrium centrifugation, HCV-RNA positive fractions separated after the treatments were further examined for immunoprecipitation with anti-core hepatitis C virus antibody, We separated hepatitis C virus particle populations according to the density difference on 35% sucrose with centrifugation, The proportions of high and low density particles in hepatitis C virus populations were determined by means of competitive reverse transcription and polymerase chain reaction. Circulating hepatitis C virus particles in chronically infected patients could be separated into two populations: those immunoglobulin-bound with high densities and -unbound with low densities, Patients with severe liver inflammation had high-density hepatitis C virus that did not precipitate with anti-immunoglobulins but with anti-core hepatitis C virus antibodies, Thus, hepatitis C virus particle populations consist of low density virions and high-density immune complexes and/or nucleocapsids. Among the chronic hepatitis patients, the dominant population shifted from low-density to high-density particles with the progression of liver disease. In acute hepatitis patients, this density shift was observed with alanine aminotransferase normalizations. Therefore, the major hepatitis C virus populations change from virion to immune complex and/or nucleocapsid with the progression of liver disease or inflammation.

引用

页码：440 / 448

页数：9

共 36 条

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