FATTY ACYLATION PROMOTES FUSION OF TRANSPORT VESICLES WITH GOLGI CISTERNAE

被引：116

作者：

PFANNER, N ^{[1
]}

GLICK, BS ^{[1
]}

ARDEN, SR ^{[1
]}

ROTHMAN, JE ^{[1
]}

机构：

[1] PRINCETON UNIV,DEPT BIOL,PRINCETON,NJ 08544

来源：

JOURNAL OF CELL BIOLOGY | 1990年 / 110卷 / 04期

关键词：

D O I：

10.1083/jcb.110.4.955

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Two different methods, stimulation of transport by fatty acyl-coenzyme A (CoA) and inhibition of transport by a nonhydrolyzable analogue of palmitoyl-CoA, reveal that fatty acylation is required to promote fusion of transport vesicles with Golgi cisternae. Specifically, fatty acyl-CoA is needed after the attachment of coated vesicles and subsequent uncoating of the vesicles, and after the binding of the NEM-sensitive fusion protein (NSF) to the membranes, but before the actual fusion event. We therefore suggest that an acylated transport component participates, directly or indirectly, in membrane fusion.

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页码：955 / 961

页数：7

相关论文

共 25 条

[1] RECONSTITUTION OF THE TRANSPORT OF PROTEIN BETWEEN SUCCESSIVE COMPARTMENTS OF THE GOLGI MEASURED BY THE COUPLED INCORPORATION OF N-ACETYLGLUCOSAMINE [J].

BALCH, WE ;

DUNPHY, WG ;

BRAELL, WA ;

ROTHMAN, JE .

CELL, 1984, 39 (02) :405-416

[2] SEQUENTIAL INTERMEDIATES IN THE PATHWAY OF INTERCOMPARTMENTAL TRANSPORT IN A CELL-FREE SYSTEM [J].

BALCH, WE ;

GLICK, BS ;

ROTHMAN, JE .

CELL, 1984, 39 (03) :525-536

[3]

BALCH WE, 1985, ARCH BIOCHEM BIOPHYS, V4240, P414

[4] VESICULAR TRANSPORT BETWEEN THE ENDOPLASMIC-RETICULUM AND THE GOLGI STACK REQUIRES THE NEM-SENSITIVE FUSION PROTEIN [J].

BECKERS, CJM ;

BLOCK, MR ;

GLICK, BS ;

ROTHMAN, JE ;

BALCH, WE .

NATURE, 1989, 339 (6223) :397-398

[5] PURIFICATION OF AN N-ETHYLMALEIMIDE-SENSITIVE PROTEIN CATALYZING VESICULAR TRANSPORT [J].

BLOCK, MR ;

GLICK, BS ;

WILCOX, CA ;

WIELAND, FT ;

ROTHMAN, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7852-7856

[6]

CIARDELLI T, 1981, LIEBIGS ANN CHEM, P828

[7] VESICLE FUSION FOLLOWING RECEPTOR-MEDIATED ENDOCYTOSIS REQUIRES A PROTEIN ACTIVE IN GOLGI TRANSPORT [J].

DIAZ, R ;

MAYORGA, LS ;

WEIDMAN, PJ ;

ROTHMAN, JE ;

STAHL, PD .

NATURE, 1989, 339 (6223) :398-400

[8] POSSIBLE ROLE FOR FATTY ACYL-COENZYME-A IN INTRACELLULAR PROTEIN-TRANSPORT [J].

GLICK, BS ;

ROTHMAN, JE .

NATURE, 1987, 326 (6110) :309-312

[9] ROLE OF AN N-ETHYLMALEIMIDE-SENSITIVE TRANSPORT COMPONENT IN PROMOTING FUSION OF TRANSPORT VESICLES WITH CISTERNAE OF THE GOLGI STACK [J].

MALHOTRA, V ;

ORCI, L ;

GLICK, BS ;

BLOCK, MR ;

ROTHMAN, JE .

CELL, 1988, 54 (02) :221-227

[10] INVOLVEMENT OF GTP-BINDING G PROTEINS IN TRANSPORT THROUGH THE GOLGI STACK [J].

MELANCON, P ;

GLICK, BS ;

MALHOTRA, V ;

WEIDMAN, PJ ;

SERAFINI, T ;

GLEASON, ML ;

ORCI, L ;

ROTHMAN, JE .

CELL, 1987, 51 (06) :1053-1062