PHOSPHOLIPID-SYNTHESIS BY EXTRACELLULAR PHOSPHOLIPASE-A2 IN ORGANIC-SOLVENTS

被引:34
作者
PERNAS, P [1 ]
OLIVIER, JL [1 ]
LEGOY, MD [1 ]
BEREZIAT, G [1 ]
机构
[1] UNIV TECHNOL COMPIEGNE,TECHNOL ENZYMAT LAB,F-60200 COMPIEGNE,FRANCE
关键词
D O I
10.1016/0006-291X(90)92369-B
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The catalytic activity of extracellular phospholipase A2 was studied in low polarity solvents where hydrolytic enzymes have been demonstrated to catalyze synthesis reactions. It was demonstrated that extracellular phospholipase A2 can catalyze the esterification of lysophosphatidylcholine with oleic acid. Up to 6.5% of lysophosphatidylcholine can be esterified into phosphatidylcholine. This activity requires a preincubation of the enzyme in a pH 9 aqueous solution containing calcium, before the incubation in the non-aqueous solvent. No transfer of fatty acid between a phospholipid and a lysophospholipid or between two phospholipids was observed. These results may be useful in understanding the function of the membrane phospholipase A2 which may catalyze acylation or deacylation depending on the local physico-chemical environment. © 1990.
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页码:644 / 650
页数:7
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