核磁共振在多糖结构解析中的应用

被引：0

作者：

王林强

机构：

[1] 华东师范大学

关键词：

核磁共振; 多糖; 结构解析;

D O I：

暂无

年度学位：

2013

学位类型：

硕士

导师：

胡炳文;

摘要：

从灵芝中提取的多糖和糖蛋白具有降低血糖的功能,其中提取的抑制高血糖的大分子FYGL可以作为治疗糖尿病的候选药物。FYGL是复杂的混合物,为进一步阐明结构性能关系,FYGL被进一步分离纯化,其中第一部分(FYGL-1)是中性多糖,第二部分(FYGL-2)是超分支中性糖蛋白,第三部分(FYGL-3)是酸性糖蛋白。仅依靠化学分析手段如高碘酸盐氧化、smith降解和甲基化分析等不能解决复杂大分子的结构信息,尤其是糖残基之间的连接顺序问题。本论文主要依靠核磁共振方法结合甲基化分析等化学分析手段,分别对这三个纯化部分进行了详细的结构分析与阐明。我们通过一维和二维核磁共振技术可以对多糖中各糖残基的13C、1H进行化学位移归属,依据二维NOESY和gHMBC谱可以获得糖残基的连接顺序,获得完整的结构信息。

引用

页数：54

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